The resulting cycle performances from our previous studies are compared for operation in 50% depth-of-discharge (DoD) and 17.5% DoD at 50% state-of-charge (SoC) cycling profiles, representing two working modes: high load and partial state-of-charge, respectively. This comparative analysis examines test cells configured with negative electrode as the capacity-limiting component. This comparative analysis, based on prior independent studies, highlights the distinct degradation mechanisms influencing negative electrode sulfation, morphology, and electrochemical behavior between the two cycling regimes. While the 50% DoD profile creates more overall sulfation due to frequent discharge to half of rated capacity, it produces greater evenness in PbSO₄ distribution across the electrode cross-sectional area. In contrast, the 17.5% DoD profile, with lower charge/discharge loads in each cycle of a test unit, exhibits an uneven distribution of PbSO₄ mostly concentrated at the edge region and larger crystal sizes due to sustained undercharge. Cyclic voltammetry reveals that the uneven sulfation in the 17.5% DoD profile results in increased hydrogen evolution reaction despite less overall degradation. This comparative insight suggests different practical optimization strategies for each operational mode, with periodic recovery charges at low current being particularly beneficial for long-term battery durability in partial state-of-charge applications.